FastQCFastQC Report
Sun 20 Nov 2022
CO_044-COL.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_044-COL.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12246
Sequences flagged as poor quality0
Sequence length25-324
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC5524.507594316511514No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5254.287114159725625No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG4974.058468071206925No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG4703.837987914421036No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC3672.9968969459415318No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG3582.9234035603462356No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG3212.621264086232239No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA2592.1149763187979747No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG2311.8863302302792748No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG2301.8781642985464642No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG2291.8699983668136535No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG2231.8210027764167893No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA2181.7801731177527356No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2111.7230115956230605No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA2091.706679732157439No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1981.6168544830965212No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1881.5351951657684142No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT1721.4045402580434427No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1591.2983831455169035No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1531.2493875551200393No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC1501.224889759921607No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT1481.2085578964559858No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC1411.1513963743263105No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT1411.1513963743263105No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC1381.1268985791278785No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1311.0697370569982034No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC1271.0370733300669606No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1190.9717458762044748No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1180.9635799444716643No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1160.9472480810060429No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1150.9390821492732321No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT1130.9227502858076106No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1120.9145843540747999No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT960.7839294463498285No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT940.7675975828842071No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA890.7267679242201535No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG870.7104360607545321No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG860.7022701290217214No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT820.6696064020904785No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT810.6614404703576677No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA780.6369426751592357No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT740.6042789482279929No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC710.5797811530295607No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT690.5634492895639392No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC680.5552833578311285No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG660.5389514943655072No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG650.5307855626326964No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT630.514453699167075No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC630.514453699167075No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG610.4981218357014535No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG570.46545810877021065No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT570.46545810877021065No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT570.46545810877021065No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG560.45729217703739994No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG560.45729217703739994No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG550.4491262453045893No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG550.4491262453045893No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG540.44096031357177856No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA540.44096031357177856No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT530.4327943818389678No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT530.4327943818389678No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT520.42462845010615713No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT520.42462845010615713No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG500.4082965866405357No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT490.4001306549077249No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA460.37563285970929283No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC450.3674669279764821No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA450.3674669279764821No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT430.3511350645108607No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG380.3103054058468071No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG340.27764167891556424No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC330.2694757471827536No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT320.2613098154499428No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG310.25314388371713215No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC280.22864608851869997No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC280.22864608851869997No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT240.19598236158745713No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA230.18781642985464642No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT210.17148456638902498No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG200.16331863465621427No Hit
CCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT200.16331863465621427No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGG180.14698677119059284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)